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| Oliveira,F.D.; Sangalli,J.R.; Meirelles,F.V.; Perecin,F.; Silva Filho,P.P.C.; Watanabe,Y.F.; Miglino,M.A.; Assis Neto,A.C.. |
| In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of the epiblast and hypoblast. The aim of this study was to ultrastructurally characterize the bovine embryo from different in vitro production techniques, with emphasis on trophectoderm and inner cell mass cells. Bovine embryos on day 7 (conception = D1) of pregnancy, derived via in vitro production techniques, were fixed for light and transmission electron microscopy processing. Results suggested that embryos produced by nuclear transfer of somatic cells and parthenogenesis showed significant changes in macroscopic and microscopic structure.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Blastocyst; In vitro production; Ultrastructure. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601483 |
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| Coelho Teixeira,Álvaro Aguiar; Simões,Manuel de Jesus; Wanderley Teixeira,Valéria; Soares Júnior,José Maria. |
| In this paper we evaluated the morphologic and quantitative aspects of the implantations sites in uteruses of female pinealectomized and/or submitted to the constant illumination rats. The experimental groups were: I - normal female rats maintained in a clear/dark cycle of 12/12 hours, for two months, mated and euthanasiated in the 6th day of pregnancy (control); II - female sham-pinealectomized rats maintained in a clear/dark cycle of 12/12 hours, for two months, mated and euthanasiated in the 6th day of pregnancy (control); III - female pinealectomized rats, maintained in a clear/dark cycle of 12/12 hours, for two months, mated and euthanasiated in the 6th day of pregnancy; IV - normal female rats maintained in constant illumination for two months, mated... |
| Tipo: Journal article |
Palavras-chave: Pinealectomy; Constant illumination; Implantation; Blastocyst. |
| Ano: 2004 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022004000300003 |
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| Sepúlveda,Soledad J; Portella,Jimmy R; Noriega,Luis P; Escudero,Ernesto L; Noriega,Luis H. |
| The aim of this study was to review the experience and outcomes of assisted reproduction cycles with embryos grown up to day 5 of development, comparing different parameters according to the ages of the patients. We retrospectively studied 1,874 assisted reproduction cycles where embryo culture was extended up to the fifth or sixth day of development. All IVF and ICSI cycles were included, comparing, according to patient age, the following rates: blastocyst formation, pregnancy, implantation and abortion. As control, we analyzed cycles with donated oocytes from young donors (OD). The number of embryos reaching the blastocyst stage is similar in all groups of patients. Only the OD group was different in terms of blastocyst formation, pregnancy and... |
| Tipo: Journal article |
Palavras-chave: Blastocyst; Long-term culture; Pregnancy rate; Implantation rate. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200012 |
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| Catena,M; Teruel,M; Morán,P; Chiapparrone,M; Echevarria,H; Soto,P. |
| Bovine campylobacteriosis caused by Campylobacter fetus is associated with reproductive losses. The knowledge about the mechanisms of bacterial pathogenesis is limited, then a murine experimental model is proposed. BALB/c females and males were used. Two-cell embryos were cultured in Ham-F10 as control group (CG). Treatment groups were constituted by the addition of Cfv 1 and 3, or Cff 2 and 5. Morulae were placed in Ham-F10 (CG); treatment groups were constituted by the addition of Cfv27, CFF (cell-free filtrate) and Brucella broth (BB). Blastocysts were cultured in MEM (CG); challenge group were constituted by the addition of Cfv 27. Differentiation, hatching, hatched, adhesion and expansion were evaluated. Results were analyzed by Chi2 test. In two-cell... |
| Tipo: Journal article |
Palavras-chave: Campylobacteriosis; Blastocyst; Morulae; In vitro; Mouse. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400004 |
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| Villamil,Paula Rodriguez; Ongaratto,Felipe Ledur; Silva,Daniela Scherer da; Rodrigues,Berenice de Avila; Rodrigues,Jose Luiz. |
| The aim of this study was to determine the effect of dimethylformamide (DF) associated with ethylene glycol (EG) or 1-2 propanediol (PROH) during vitrification, on the in vitro development of mouse blastocysts. Cryoprotectant toxicity was evaluated exposing embryos into three different equilibrium solutions (ES) composed by DF, EG or PROH mixtures (10% v/v of each) in mPBS + 0.5% PVA at different interval times (1, 3 and 10min). In a second experiment, embryos were exposed to the same ES (either 1 or 3min), following for the three respectively vitrification solutions (VS) (20% v/v of each) for 30s. After 72 hours of in vitro culture, embryo hatching and expansion rates were similar for the ES1 and ES2 equilibration solutions during the time interval of 1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mouse; Blastocyst; Vitrification; Dimethylformamide; Glass micro pipettes. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011001100022 |
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